Lane Labs
Lane Labs
DNA gel electrophoresis: protein contamination & the results?
In the laboratory of molecular biology I, we tried to isolate genomic DNA and cut using restriction enzymes to hybridize with the plasmid. However, when we tested digest with gel electrophoresis, I got strange results. Path to the cut DNA (control) has two bands: one band works very slow and vague, while other bands are brighter and more quickly. Another path has been cut DNA (digests) which has a band that is in the same position of the band more quickly in the DNA is cut. I'm having trouble interpreting the results. Band is slower in the lane cut the RNA or protein? Is there any reason why it does not appear on the cut line? And how come the DNA is cut and the DNA was cut bands that have the same length?
Are the two DNA that you run The second plasmid DNA? I assume so, because genomic DNA would look like a smear, especially when cut. So for now I will assume that you cut plasmids show 2 bands. It's actually quite normal, because the plasmid will be present in three forms, supercoiled (CCC form), open circular (oc form) and linear (l) form. L form is the result violates DNA during isolation, and in an ideal world you want your plasmid DNA to be mainly from the CCC form. Usually ccc forms run faster than linearly, but it's hard to say from your information what happens. So it looks as though you've broken plasmid, before it was cut.
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