10Aug/09Off
Cell Power
Cell Power
how to find hte actual size of a cell in low power?
How to find the actual size of a cell? in low power, and then convert it to find the size of it in high/medium power, please give me the formula?
Under low (100X) power focus on a transparent metric ruler.
Measure the diameter of the microscopic field by seeing how many mm markings you see. Let's say that the diameter measures 2mm across; That's 2000 micrometers (or microns).
Remove the ruler. Now you know the diameter of the field (2000
microns). If you think that you can fit 10 cells from one end of the field to the other, then, 2000 microns/10 = 200 microns = the estimated size of each cell.
When you switch from low (100X) to high (400X) power, the diameter of the field is 100/400= 1/4 as long.
1/4 X 2000 microns = 500 microns. The diameter of the field in high power is 500 microns.
By the way, when you change from low to high power or visa-versa, the size of your cell does not change. The only change is the diameter of the field of view.
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